Reconstitute your peptides in 4 steps
Materials, step-by-step technique, dose calculator and storage — all in one place, without unnecessary jargon.
The 4 essential steps
Same protocol for all peptides. Follow this order — the calculator will then tell you how much to draw on the syringe.
Gather your materials
Peptide vial, bacteriostatic water (BAC), 1 mL syringe, alcohol swabs.
Inject water slowly
Add BAC along the inside wall of the vial — never directly onto the powder.
Mix without shaking
Roll the vial between your palms. Wait 1–2 minutes for the powder to dissolve.
Calculate your dose
Use the calculator below: enter your vial (mg), water added (mL) and target dose (mcg).
Need more detail? The full 6-step protocol and FAQ are further down this page.
Reconstitution by peptide
Select your peptide: indicative volumes pre-filled in the calculator. You can adjust for your protocol.
Reconstitution calculator
Enter the 3 values below to know how much to draw on your syringe.
1. How much peptide is in your vial?
2. How much BACT-H2O will you add?
3. What dose do you want per injection?
Result
Draw on your insulin syringe:
10
units
(soit 0.10 mL)
20
total doses in vial
2500
mcg per mL of solution
* Indicative results for in vitro research. Not medical advice. Products for scientific use only.
What is reconstitution?
Your peptide arrives in lyophilized form: a white powder in a vial. Lyophilization (freeze-drying) allows the product to be stored for months or years without degradation.
Reconstitution involves adding BACT-H2O to transform this powder into a liquid solution. It's a simple but important step: proper technique ensures a stable, usable solution.
In summary
Powder + BACT-H2O = ready-to-use solution. The process takes about 3 minutes.
The materials you need
Gather everything before you start. Available at pharmacies or on our site.
Your vial
Lyophilized peptide (white powder)
The powder is dehydrated for long-term storage. Reconstitution turns it into an injectable solution.
BACT-H2O
Sterile solvent (10 ml)
Contains 0.9% benzyl alcohol to limit bacterial growth. Keeps the reconstituted solution for 28 days in the fridge.
Insulin syringe
1 mL, U-100 graduated
Each small mark = 0.01 mL (10 µL). Ideal for precise doses. Available at pharmacies without prescription.
Where to find the materials?
BACT-H2O and insulin syringes: at pharmacies, often without prescription. You can also add BACT-H2O to your order on our site.
The 6 steps in detail
Detailed protocol to go further — hygiene, volume precision and key watchpoints.
Step 1
Prepare your workspace
Hygiene and organization
- 1Wash your hands thoroughly.
- 2Prepare a clean, disinfected surface (workbench, table).
- 3Lay out all materials: vial, BACT-H2O, syringe, alcohol swabs.
Working in a calm place avoids mistakes. Take your time.
Step 2
Disinfect the vial cap
Crucial step to avoid contamination
- 1Open an alcohol swab (70% isopropyl alcohol).
- 2Gently wipe the rubber cap of the vial.
- 3Let it air dry for 30 seconds. Do not blow on it.
A well-disinfected cap limits the risk of bacterial contamination.
Step 3
Draw the BACT-H2O
Volume precision
- 1Remove the needle cap from the syringe.
- 2Draw air into the syringe up to the desired volume (e.g. 1 mL).
- 3Insert the needle into the water vial cap, inject the air.
- 4Invert the vial, slowly draw the water up to the graduation.
Injecting air makes drawing easier. Never force.
Step 4
Inject water into the peptide vial
The actual reconstitution
- 1Insert the needle into the peptide vial cap.
- 2Inject the water slowly along the inner wall, never directly onto the powder.
- 3Remove the syringe and needle.
A direct stream onto the powder can create bubbles and denature the peptide.
Step 5
Mix gently
Without shaking
- 1Never shake the vial.
- 2Roll the vial between your palms or tilt it gently.
- 3The powder should dissolve in 1 to 2 minutes. The solution should be clear or slightly opalescent.
Shaking creates shear forces that can degrade the protein.
Step 6
Check and store
Quality control
- 1Verify there are no visible particles or undissolved residue.
- 2If the solution is cloudy or contains particles, do not use.
- 3Store in the refrigerator (2–8 °C) in its original packaging.
Clear solution = ready to use. Cloudy solution = do not use.
Never shake
Shear forces can denature the protein. Mix only by gentle rolling between your palms.
Storage: before and after
Before reconstitution
- −20 °C (freezer): 24 to 36 months
- 4 °C (refrigerator): up to 6 months
- Keep away from light and moisture
After reconstitution
- 2–8 °C: 28 days with BACT-H2O
- 2–8 °C: 7 days with sterile water
- Never refreeze
FAQ
All our peptides, 98.64% purity
GLP-3 RTA 20MG, GHK 50MG, MT2, BPC 10MG, TB 10MG, TSM 10MG. Fast delivery in Europe. Card or bank transfer. Documented third-party testing.